Insertion junction: LMJ.RY0402.132661_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.132661
Chromosome:chromosome 9
Location:1854439
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g395750 (1 of 6) 3.6.3.43 - Peptide-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTTTGTTTGGGGTTGGTTTTGGATCTGG
Internal bar code:GCTCGCGCGAAGAACTGAGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:235
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:160
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTTGTGCTTTCTGGGGT
Suggested primer 2:CGCAAACACGTCTCAAAGAA