| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132662 |
| Chromosome: | chromosome 6 |
| Location: | 5333034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283350 | MRPS34,mS34 | (1 of 1) K17412 - small subunit ribosomal protein S34 (MRPS34); Mitochondrial ribosomal protein S34 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATGCCGCTCCTTTGCGGAGCACATTGT |
| Internal bar code: | GTAACTTGGCTTCCCCGTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 99.6189 |
| LEAP-Seq n confirming: | 12808 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTAAGTGCAAGGGGCCTG |
| Suggested primer 2: | CCCTACCCAGCTAATCCTCC |