| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132667 |
| Chromosome: | chromosome 13 |
| Location: | 2569523 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580950 | GT90-25,GT90F25 | (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 25 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTCGCATTTGGGCAGGAACCTGTTGGT |
| Internal bar code: | TATCGGGGCCCGATCCTCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 99.7744 |
| LEAP-Seq n confirming: | 2211 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCTAGACGGAAGCTGG |
| Suggested primer 2: | GTGTGGTCCCGACAAGAACT |