| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132696 |
| Chromosome: | chromosome 14 |
| Location: | 3421691 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630600 | (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCAGCACGAACAGCTGCATGGCAGCACT |
| Internal bar code: | TACGACACGGTCCGGCAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 21.1554 |
| LEAP-Seq n confirming: | 1604 |
| LEAP-Seq n nonconfirming: | 5978 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCGTCATGTCATACACA |
| Suggested primer 2: | ACTGTGGAGCCGATGCTAGT |