| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132706 |
| Chromosome: | chromosome 7 |
| Location: | 5191336 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348550 | TGL13 | (1 of 3) PTHR21493:SF153 - PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGCACGGAGACCCTCGAGCCACTTGCA |
| Internal bar code: | TTGGTTGCGGGAACTGCTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 52.1949 |
| LEAP-Seq n confirming: | 3139 |
| LEAP-Seq n nonconfirming: | 2875 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGGTCATGTTGCCGCTT |
| Suggested primer 2: | CCCGTGAGACTTTTGTGGTT |