| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132719 |
| Chromosome: | chromosome 6 |
| Location: | 5622680 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285900 | FAP396 | (1 of 2) IPR000104//IPR003590 - Antifreeze protein, type I // Leucine-rich repeat, ribonuclease inhibitor subtype; Flagellar Associated Protein 396 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTCAGGCAGCAGCAGTGGATGTGGTG |
| Internal bar code: | GCGCCGGAGCTGCACCGCGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 63.4081 |
| LEAP-Seq n confirming: | 2374 |
| LEAP-Seq n nonconfirming: | 1370 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCACCCATTCCCATTAG |
| Suggested primer 2: | GGACGAGTGGAAGCTGAGTC |