| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132723 |
| Chromosome: | chromosome 2 |
| Location: | 4552685 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100800 | (1 of 1) PTHR22847//PTHR22847:SF410 - WD40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 27 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCAGCACCCCAGTCCCACACCGCATCC |
| Internal bar code: | CCAGCGAGCAGCTGTATGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 176 |
| LEAP-Seq percent confirming: | 58.7664 |
| LEAP-Seq n confirming: | 1210 |
| LEAP-Seq n nonconfirming: | 849 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGGGCATACATACCTGG |
| Suggested primer 2: | GTGAACACCAGCCCAAAGTT |