| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132751 |
| Chromosome: | chromosome 16 |
| Location: | 6349047 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674100 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCGCCGCGCGCGATCGCCGGAAGGGAC |
| Internal bar code: | GTACGGGATGGAACGGGGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 92.8204 |
| LEAP-Seq n confirming: | 3245 |
| LEAP-Seq n nonconfirming: | 251 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGACACGAGGATTCGAC |
| Suggested primer 2: | AGTGCGTGTGACGAAGACAG |