| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132768 |
| Chromosome: | chromosome 1 |
| Location: | 915863 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004900 | Similar to Putatative Protease/Amidase; (1 of 1) PTHR11019//PTHR11019:SF1//PTHR11019:SF97 - THIJ/PFPI // SUBFAMILY NOT NAMED // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCCATGATGTGGAGTGCGAAGTAAGTT |
| Internal bar code: | AATCATGGTCAGCGACGGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.7266 |
| LEAP-Seq n confirming: | 4013 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGGGACAAGCTCGTAT |
| Suggested primer 2: | GCCTCTCCACCTCTGTCAAG |