| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132810 |
| Chromosome: | chromosome 14 |
| Location: | 3657824 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631400 | (1 of 80) IPR003882 - Pistil-specific extensin-like protein | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAGCATGACTTCCGGCTATTGCGGCAG |
| Internal bar code: | AGCTTTCTTAAAAGATAGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 375 |
| LEAP-Seq percent confirming: | 99.5831 |
| LEAP-Seq n confirming: | 1911 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTTCTTGGGGTTTTGG |
| Suggested primer 2: | GAACACGCACTGCGGTAGTA |