| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132817 |
| Chromosome: | chromosome 1 |
| Location: | 1508299 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007737 | FBT1 | Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTCCAACCTACACGTCTACAGCAGCAC |
| Internal bar code: | ACCACCGTACGTCTCCGCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.0683 |
| LEAP-Seq n confirming: | 638 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAAGCTGGATAGG |
| Suggested primer 2: | TGGAAAATGGAGATTCGGAG |