Insertion junction: LMJ.RY0402.132821_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.132821
Chromosome:chromosome 3
Location:9218873
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g213649 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGCAGGGCTGTGAGACACCTCATCCGC
Internal bar code:CTTGTGCAGGTGGGTCCTAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:178
LEAP-Seq percent confirming:99.209
LEAP-Seq n confirming:5268
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGGGCGACATCAGTTTT
Suggested primer 2:CAAACACCCTGCTCTCAGGT