| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132823 |
| Chromosome: | chromosome 12 |
| Location: | 4345551 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520150 | SPLH3,SPL23 | (1 of 3) K12818 - ATP-dependent RNA helicase DHX8/PRP22 (DHX8, PRP22); DEAD/DEAH box helicase, possible nuclear pre-mRNA splicing factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATTGGGGGTAAACTCTCCCCCACCCC |
| Internal bar code: | GGGTTAACAGTAACCTTGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 437 |
| LEAP-Seq percent confirming: | 99.7096 |
| LEAP-Seq n confirming: | 2060 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGAGTGGGTGTAACGGAA |
| Suggested primer 2: | TCTTGGCATCTATTGGGCTC |