| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132827 |
| Chromosome: | chromosome 14 |
| Location: | 4006279 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633350 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCGCACCGAGCTGCAGGCCGCCCGCCA |
| Internal bar code: | GCTCCCATACGAGCTTCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 51.5264 |
| LEAP-Seq n confirming: | 1671 |
| LEAP-Seq n nonconfirming: | 1572 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGAGGAGGGAAGAGGG |
| Suggested primer 2: | GGGCTTTGCACACCACTTAT |