| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132829 |
| Chromosome: | chromosome 6 |
| Location: | 5173615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281563 | (1 of 62) PF00226 - DnaJ domain (DnaJ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGAAGGTGAGCGGTGGCGACGGGGTTC |
| Internal bar code: | GAACGATCGGAAAGGAGTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 94.9907 |
| LEAP-Seq n confirming: | 3053 |
| LEAP-Seq n nonconfirming: | 161 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTCAACGACTCAGCACA |
| Suggested primer 2: | CTGCATGCTTCAATGCTTGT |