Insertion junction: LMJ.RY0402.132845_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.132845
Chromosome:chromosome 4
Location:515707
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217935 (1 of 1) K11886 - proteasome component ECM29 (ECM29) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACGCCCCGTACGACACGTGCGCCCTCC
Internal bar code:AAGAATGACGCTGGGCGCCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGCGCATACCGTATTTCT
Suggested primer 2:GAGTCGTGTCGTCCTTGTCA