| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132851 |
| Chromosome: | chromosome 6 |
| Location: | 8603926 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g308950 | (1 of 2) PF02453 - Reticulon (Reticulon) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGTGTGTGTGCCCCGTGACTTTCGAC |
| Internal bar code: | ATCGCGGGAAAATGTTACGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 99.587 |
| LEAP-Seq n confirming: | 4340 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACACAAAACCCTGCCTGC |
| Suggested primer 2: | TGCCCCCTTCTGTAAGATTG |