| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132867 |
| Chromosome: | chromosome_8 |
| Location: | 1914025 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g367250 | FAP176 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCGGCATGGCAGATTGTTGCATTATGGC |
| Internal bar code: | CTTAACCTGGCCTCGGCGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 99.1622 |
| LEAP-Seq n confirming: | 3551 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAACGAGCGGATTAGCAG |
| Suggested primer 2: | TGGTGAGAGCAGTGTTGAGG |