| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.132869 |
| Chromosome: | chromosome 2 |
| Location: | 4147426 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098050 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGCCAGCAGGTGGGACAGCTGTGTGT |
| Internal bar code: | GCGCCGCGCCAGAAGCTTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 284 |
| LEAP-Seq percent confirming: | 99.2761 |
| LEAP-Seq n confirming: | 4114 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTGTCTCGCGTGAGGAG |
| Suggested primer 2: | GGCCCACGACTATACCTCAA |