| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132870 |
| Chromosome: | chromosome 2 |
| Location: | 6002287 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112266 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGGCAGAGGGCCGGGAAGCCCTGGTG |
| Internal bar code: | CCACCGGGCGTTTATTGGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 65.2303 |
| LEAP-Seq n confirming: | 2861 |
| LEAP-Seq n nonconfirming: | 1525 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACCTCCTGCTCTTCGTC |
| Suggested primer 2: | GAGCCCTTTCGACAGTCTTG |