| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132876 |
| Chromosome: | chromosome 11 |
| Location: | 3728079 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482900 | RAB20,RAB18C,RABC3 | Small Rab-related GTPase; (1 of 3) K07910 - Ras-related protein Rab-18 (RAB18) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTCTTGTTGGCCACCACCATCTGGATG |
| Internal bar code: | CGGCGGCACGGTTGTTAATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 765 |
| LEAP-Seq percent confirming: | 97.8459 |
| LEAP-Seq n confirming: | 8267 |
| LEAP-Seq n nonconfirming: | 182 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTGGAATAACCGACCT |
| Suggested primer 2: | AGGGCATCATCTTTGGTGAG |