| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132900 |
| Chromosome: | chromosome 12 |
| Location: | 7889231 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552700 | (1 of 2) IPR001190//IPR006626//IPR011050//IPR017448 - SRCR domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // SRCR-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTTGCTCACATACGCACACGCACATAC |
| Internal bar code: | GCTTTAACCGGTTGCGAGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.5866 |
| LEAP-Seq n confirming: | 11564 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACTTCCAAACCGCATGG |
| Suggested primer 2: | ACAAAGGTGATGCCGAAGTC |