Insertion junction: LMJ.RY0402.132903_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.132903
Chromosome:chromosome 17
Location:5087660
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g734900 (1 of 1) 3.4.17.19 - Carboxypeptidase Taq / Carboxypeptidase 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCAGACAACCATCTCCCCAGCCCTTCTC
Internal bar code:CCTTTCCTCGTGTCTATAAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:47
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TACGACAAGAAGACCGACCC
Suggested primer 2:AACATTTGCACATTACGCCA