Insertion junction: LMJ.RY0402.132915_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132915
Chromosome:chromosome 16
Location:5181866
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g683100 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTCTCTCTAGCTCTTTATAGTAGACCCG
Internal bar code:TGAATGGTTATGTAAGTGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:927
LEAP-Seq percent confirming:99.6678
LEAP-Seq n confirming:2100
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TCATACGGGCATACGAACAA
Suggested primer 2:AACCCAGTGGCGTATGAGAC