| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132922 |
| Chromosome: | chromosome 17 |
| Location: | 5679938 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738632 | RLS13 | RegA/RlsA-like protein; (1 of 10) IPR010919 - SAND domain-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGAGTGTAGTCAACATCAAATGAAAG |
| Internal bar code: | GGTGGCCTTTTAACAAAAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 851 |
| LEAP-Seq percent confirming: | 99.6036 |
| LEAP-Seq n confirming: | 143221 |
| LEAP-Seq n nonconfirming: | 570 |
| LEAP-Seq n unique pos: | 245 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGCGTCTTAAATTCTGC |
| Suggested primer 2: | TGCATGTTTGTGAAGGGTGT |