| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.132930 |
| Chromosome: | chromosome 2 |
| Location: | 4746854 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102400 | RPC1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCATGCGAGTACGATGCTGGCTCATTG |
| Internal bar code: | GGTTTTTTGATCGAGCGGGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 99.7952 |
| LEAP-Seq n confirming: | 4873 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGAAATTGCTTGTTGGG |
| Suggested primer 2: | TTCACTACAACCTCAGCCCC |