| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132931 |
| Chromosome: | chromosome 4 |
| Location: | 1512311 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213750 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCAAGCACGCCGCAGCGCGGCAGACC |
| Internal bar code: | GGGGCCTCCGTCGAATGTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 99.9337 |
| LEAP-Seq n confirming: | 3013 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCGGAGGTAAGGGTGTT |
| Suggested primer 2: | GGACAAGAGGGACAGCTCAG |