| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132951 |
| Chromosome: | chromosome 14 |
| Location: | 3535691 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630895 | (1 of 1) PTHR13789:SF90 - FAD-BINDING MONOOXYGENASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCACGCCACCGTCCACGGTCCCACGGTC |
| Internal bar code: | GGGAAGAGCAAGGGGAGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 443 |
| LEAP-Seq percent confirming: | 82.3619 |
| LEAP-Seq n confirming: | 5921 |
| LEAP-Seq n nonconfirming: | 1268 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGGCTAGGACTCACTG |
| Suggested primer 2: | GCCACAAACACAAACACACA |