| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.132953 |
| Chromosome: | chromosome_11 |
| Location: | 2625143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476376 | FAP221 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGTGTTAGCCATCCGCTGACCAGCATGGC |
| Internal bar code: | CCACGAACCTAGTCCCTCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 97.9128 |
| LEAP-Seq n confirming: | 5770 |
| LEAP-Seq n nonconfirming: | 123 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGCTGGACTTCGTTTG |
| Suggested primer 2: | CAATTCGCTCTGCACCTGTA |