Insertion junction: LMJ.RY0402.132961_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.132961
Chromosome:chromosome 2
Location:3241126
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTTCGCCGCCGTCACCGCCTTCAACGC
Internal bar code:ACCCTAGGAGTGGTTCGGGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:337
LEAP-Seq percent confirming:97.4648
LEAP-Seq n confirming:13686
LEAP-Seq n nonconfirming:356
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCGCCCTTCTATATACT
Suggested primer 2:GTGTACGAAGGAGTGCCCAT