| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.132970 |
| Chromosome: | chromosome 6 |
| Location: | 4894871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279183 | GEX150 | (1 of 1) PF09808 - Small nuclear RNA activating complex (SNAPc), subunit SNAP43 (SNAPc_SNAP43) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAAATGCAGTCAGTAATGATGGAGGAG |
| Internal bar code: | ATAAGACACGGGGCGCAGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 98.2779 |
| LEAP-Seq n confirming: | 4223 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGACTGGCAACACCTT |
| Suggested primer 2: | CAAGCGCAGAGTGTCAGAAG |