| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133010 |
| Chromosome: | chromosome 17 |
| Location: | 6080047 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741300 | SAK1 | Regulator of acclimation to singlet oxygen | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCATCGCCACCTGACTCCGCTGTGGCAT |
| Internal bar code: | TATTCGGGGCAACGAGTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 99.2558 |
| LEAP-Seq n confirming: | 9603 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAAAATCTGAAGATGGGC |
| Suggested primer 2: | TCCAGGCTGTGTTGCTACTG |