| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133025 |
| Chromosome: | chromosome 17 |
| Location: | 4890639 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCGCTGGCGCGCTGCAGGCCGGCGTCC |
| Internal bar code: | CGTGTCCCCGGGTAAAAGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 94.1006 |
| LEAP-Seq n confirming: | 7130 |
| LEAP-Seq n nonconfirming: | 447 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATAACCAACTACGCGAT |
| Suggested primer 2: | TCTGTGCGGTGAGCATAGTC |