| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133029 |
| Chromosome: | chromosome 1 |
| Location: | 7570160 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054600 | (1 of 8) IPR000157 - Toll/interleukin-1 receptor homology (TIR) domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTGCGGCCACGCGTCCGTGTGTGCGTC |
| Internal bar code: | CGGCCGAGCCGTTAACTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 25 |
| LEAP-Seq percent confirming: | 95.7237 |
| LEAP-Seq n confirming: | 291 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTGCACCACTGTTGAGG |
| Suggested primer 2: | CTCACCACGATGGTCACAAC |