Insertion junction: LMJ.RY0402.133039_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133039
Chromosome:chromosome 17
Location:6214728
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g742050 (1 of 18) IPR011051 - RmlC-like cupin domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAACACAATATTAGACCAACAACATGTTT
Internal bar code:TTAATGGCCTCGAACCCCCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:161
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCCTGGTACGTGTGGAC
Suggested primer 2:TCATGAGCACGTCGATAAGC