Insertion junction: LMJ.RY0402.133054_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133054
Chromosome:chromosome 2
Location:5399965
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g107350 DHC4 (1 of 3) IPR003593//IPR004273//IPR011704//IPR013602//IPR024317//IPR024743//IPR026983//IPR027417 - AAA+ ATPase domain // Dynein heavy chain domain // ATPase, dynein-related, AAA domain // Dynein heavy chain, domain-2 // Dynein heavy chain, P-loop containing D4 domain // Dynein heavy chain, coiled coil stalk // Dynein heavy chain // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTGCTCCACCGCGTACAGGAACATGGC
Internal bar code:CCTGGGATTTGCAATACGCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:661
LEAP-Seq percent confirming:96.6629
LEAP-Seq n confirming:2549
LEAP-Seq n nonconfirming:88
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CAACAGTTGACAATGGACGG
Suggested primer 2:GGACCGCTCCAACAGTTTTA