Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.133055 |
Chromosome: | chromosome_1 |
Location: | 7816745 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g056696 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGGGCCCAGTACTGCTGATCGCCCTCTGC |
Internal bar code: | TTGGTCCCGGTTAAGTTTGCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 957 |
LEAP-Seq percent confirming: | 99.5261 |
LEAP-Seq n confirming: | 1890 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCCCCTGTCACAGTAGGTA |
Suggested primer 2: | CACCAGCACAGTCTTCTCCA |