Insertion junction: LMJ.RY0402.133058_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133058
Chromosome:chromosome 13
Location:3735313
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589400 PRL6 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGTGTTCGAGCATCGGAATCCCGGCTTG
Internal bar code:GTGTCGATCGGTACGACGTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:942
LEAP-Seq percent confirming:99.3957
LEAP-Seq n confirming:8718
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAAACACAGGTTGGCCTC
Suggested primer 2:GGAAACGGAAGACTGCTGAG