| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.133065 |
| Chromosome: | chromosome 3 |
| Location: | 7421471 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205950 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACGGTGGCGGAAGTGGTGGATCGACGT |
| Internal bar code: | AGGACAAATGGAGAAAGCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 744 |
| LEAP-Seq percent confirming: | 99.1211 |
| LEAP-Seq n confirming: | 6090 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGATGACCTGCAAGCAAA |
| Suggested primer 2: | GTAGGTGGCAGTAAGCCTGC |