Insertion junction: LMJ.RY0402.133094_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.133094
Chromosome:chromosome 12
Location:8420043
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g548550 (1 of 1) PF05742 - Transport and Golgi organisation 2 (TANGO2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCCAGCGCGCTCTCGCGCCAGCTGCCGT
Internal bar code:GGCACGCCAGGTGGTAACCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:514
LEAP-Seq percent confirming:95.215
LEAP-Seq n confirming:28813
LEAP-Seq n nonconfirming:1448
LEAP-Seq n unique pos:76

Suggested primers for confirmation by PCR

Suggested primer 1:GATGTAGAATGGAAGCCGGA
Suggested primer 2:GGCACCTCTACACCCCTACA