Insertion junction: LMJ.RY0402.133114_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133114
Chromosome:chromosome 17
Location:420530
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g698903 (1 of 1) IPR000014//IPR002395 - PAS domain // HMW kininogen CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCTGGCTGTTGTCCAACACGTCGATGAG
Internal bar code:TTGGCAGGGCGAGACTGGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:830
LEAP-Seq percent confirming:80.6
LEAP-Seq n confirming:14454
LEAP-Seq n nonconfirming:3479
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGACGTTGTTGTGCAGC
Suggested primer 2:GAGGCTCAACTTGGCTATGC