| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133172 |
| Chromosome: | chromosome 10 |
| Location: | 4390058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451450 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTTGCAACCCCCTGTGGCCTCACCCCC |
| Internal bar code: | CCATCAGGCTGACACCCAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 153 |
| LEAP-Seq percent confirming: | 88.0086 |
| LEAP-Seq n confirming: | 411 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCACAGAGACGTGGAACA |
| Suggested primer 2: | ACACACACACTGATCTCGCC |