| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133223 |
| Chromosome: | chromosome 13 |
| Location: | 3368642 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586800 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTGCAGGCTACGGGAACCTCGTGGGC |
| Internal bar code: | CGATGTGTGCCCGGGCCACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 99.0268 |
| LEAP-Seq n confirming: | 407 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATACGCACACAGATCCAC |
| Suggested primer 2: | GAAGACGCTGAGGTGTGTGA |