| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133230 |
| Chromosome: | chromosome 3 |
| Location: | 7673446 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203850 | ATS1 | ATP-sulfurylase; (1 of 2) 2.7.7.4 - Sulfate adenylyltransferase / Sulfurylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAAGAGCCAGGCAACCTCCTCGCGCACC |
| Internal bar code: | TATTCCTCCGGCGTCGCCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.8673 |
| LEAP-Seq n confirming: | 2258 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGCAAGCAAAATAGCGG |
| Suggested primer 2: | TACACCGAGGAGAAGGGCTA |