| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.133236 |
| Chromosome: | chromosome 17 |
| Location: | 6080912 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741300 | SAK1 | Regulator of acclimation to singlet oxygen | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGGCAGCAGCAGCAGCAACCGTGGCAGC |
| Internal bar code: | GGAAGTGAGACACGGCGATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 42.0442 |
| LEAP-Seq n confirming: | 3554 |
| LEAP-Seq n nonconfirming: | 4899 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGGAGAGGCTGAACTCG |
| Suggested primer 2: | CTACTGCTGCTACAGCCACG |