Insertion junction: LMJ.RY0402.133246_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133246
Chromosome:chromosome 16
Location:5623853
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g679700 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCCAAGCCAGTGCATCGTGGCAAGGAC
Internal bar code:GATCGACCATCAGGGCATTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:444
LEAP-Seq percent confirming:99.7227
LEAP-Seq n confirming:4316
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTGCTTCGAGCTTTTCT
Suggested primer 2:GTCTGGGGTTGAGAGGTTGA