| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133262 |
| Chromosome: | chromosome 13 |
| Location: | 3625958 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588600 | KIN1-1,KIN1A | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAAAGCGAGCGGCGAGCACCTGTGATAGC |
| Internal bar code: | TTAGGGGATCTAGGACCGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 149 |
| LEAP-Seq percent confirming: | 94.3199 |
| LEAP-Seq n confirming: | 631 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGACAGCGCTCGTATCTT |
| Suggested primer 2: | GCGTGCCATAAAAGTACCGT |