| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133296 |
| Chromosome: | chromosome 2 |
| Location: | 5255595 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106300 | (1 of 1) PTHR12883:SF0 - COILED-COIL DOMAIN-CONTAINING PROTEIN 47 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGATATGGCGCCCTACGGCGCTGCAGTG |
| Internal bar code: | TGAACGATCAACCTTTTCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 504 |
| LEAP-Seq percent confirming: | 99.687 |
| LEAP-Seq n confirming: | 5095 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCCCTTCATTATCCTGC |
| Suggested primer 2: | ATATGTTCCTTTGTTGCGGC |