Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.133298 |
Chromosome: | chromosome 6 |
Location: | 2224427 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g266276 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCCCTTGTTGCATCAATCAGGTGATGT |
Internal bar code: | CCTACGGTGTGCCCGCTTCTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1312 |
LEAP-Seq percent confirming: | 99.4089 |
LEAP-Seq n confirming: | 6391 |
LEAP-Seq n nonconfirming: | 38 |
LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCCGTTTCATTGCCTAACC |
Suggested primer 2: | CAAGACTATTGGCCCACGTT |