| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.133302 |
| Chromosome: | chromosome 3 |
| Location: | 8842871 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206817 | (1 of 2) IPR001739//IPR016177 - Methyl-CpG DNA binding // DNA-binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTGTGCTGGCGCGGTTGAACTGCCCC |
| Internal bar code: | GGTTTAGACGCAGCGGCAAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.3721 |
| LEAP-Seq n confirming: | 6963 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTTCAATGGGGAGGTTC |
| Suggested primer 2: | ATTCAGTTACGCCGAGCTGT |